Enkapsulasi Streptomyces enissocaesilis strain RFZm-Pg Potensial Plant Growth Promoting Rhizobacteria dalam Maktriks Sodium Alginate
Keywords:
Streptomyces enissocaesilis strain RFZm-Pg; sodium alginate; enkapsulasi; viabilitas inokulum; biodegradasi.Abstract
Streptomyces merupakan aktinomiset tanah yang berpotensi sebagai mikroba pemacu pertumbuhan tanaman dan agen biokontrol. Namun, keberhasilan aplikasinya di lapangan sering tidak konsisten akibat tekanan lingkungan dan rendahnya stabilitas formulasi. Penelitian ini bertujuan menganalisis kemampuan matriks natrium alginat dan kalsium klorida dalam mempertahankan viabilitas inokulum Streptomyces enissocaesilis strain RFZm-Pg. Selain itu, penelitian ini mengevaluasi karakteristik fisik enkapsul melalui pengamatan morfologi SEM, pembengkakan, kehilangan massa, dan viabilitas inokulum terenkapsulasi di tanah steril. Inokulum diperoleh dari medium beras-jagung, kemudian
disuspensikan dalam natrium alginat 2% (b/v) dan dienkapsulasi melalui gelasi ionik menggunakan CaCl₂ 0,1 M. Viabilitas inokulum dalam larutan alginat dan setelah enkapsulasi dianalisis menggunakan metode hitung cawan. Pengamatan pembengkakan dan kehilangan massa dilakukan pada tanah steril menggunakan kantong nilon. Hasil menunjukkan bahwa viabilitas inokulum dalam larutan alginat meningkat dari 1,3 × 10^7 CFU/mL pada menit ke-0 menjadi 4,5 × 10^7 CFU/mL pada menit ke-30, kemudian menurun menjadi 4,5 × 10^6 CFU/mL pada menit ke-180. Viabilitas pasca-enkapsulasi mencapai 2,5 × 10^8 CFU/g. Enkapsul menunjukkan pembengkakan awal sebesar 11,9%
pada hari ke-3 dan kehilangan massa kumulatif sebesar 70,3% setelah empat minggu. Viabilitas inokulum terenkapsulasi di tanah meningkat dari 4,7 × 10^3 menjadi 7,6 × 10^5 CFU/g selama empat minggu. Hasil ini menunjukkan bahwa matriks natrium alginat-kalsium klorida mampu mempertahankan viabilitas inokulum dan mendukung pelepasan bertahap Streptomyces enissocaesilis strain RFZm-Pg di tanah steril.
Downloads
References
Adiyatama, M. D., Mariana, M., & Budi, I. S.
(2023). Uji lapang aplikasi Trichoderma sp.
dan PGPR dalam menekan kejadian penyakit
antraknosa pada cabai rawit hiyung. Jurnal
Proteksi Tanaman Tropika, 6(3), 700–707.
https://doi.org/10.20527/jptt.v6i3.2165
Abka-khajouei, R., Tounsi, L., Shahabi, N., Patel,
A. K., Abdelkafi, S., & Michaud, P. (2022).
Structures, properties and applications of
alginats. Marine Drugs, 20(6), 364.
https://doi.org/10.3390/md20060364
Barzkar, N., Sheng, R., Sohail, M., Jahromi, S. T.,
Babich, O., Sukhikh, S., & Nahavandi, R.
(2022). Alginat lyases from marine bacteria:
An enzyme ocean for sustainable future.
Molecules, 27(11), 3375.
https://doi.org/10.3390/molecules27113375
Bashan, Y., de-Bashan, L. E., Prabhu, S. R., &
Hernandez, J. P. (2014). Advances in plant
growth-promoting bacterial inoculant
technology: Formulations and practical
perspectives (1998–2013). Plant and Soil,
378, 1–33.
Bobek, J., Šmídová, K., & Čihák, M. (2017). A
waking review: Old and novel insights into
spore germination in Streptomyces. Frontiers
in Microbiology, 8, 2205.
https://doi.org/10.3389/fmicb.2017.02205
Campbell, K. T., Stilhano, R. S., & Silva, E. A.
(2018). Enzymatically degradable alginat
hydrogel systems to deliver endothelial
progenitor cells for potential revasculature
applications. Biomaterials, 179, 109–121.
https://doi.org/10.1016/j.biomaterials.2018.0
6.038
Cao, L., Lu, W., Mata, A., Nishinari, K., & Fang,
Y. (2020). Egg-box model-based gelation of
alginat and pectin: A review. Carbohydrate
Polymers, 242, 116389.
https://doi.org/10.1016/j.carbpol.2020.11638
9
Dow, L., Gallart, M., Ramarajan, M., Law, S. R.,
& Thatcher, L. F. (2023). Streptomyces and
their specialised metabolites for
phytopathogen control – Comparative in vitro
and in planta metabolic approaches. Frontiers
in Plant Science, 14, 1151912.
https://doi.org/10.3389/fpls.2023.1151912
Galaviz, C., Lopez, B. R., de-Bashan, L. E.,
Hirsch, A. M., Maymon, M., & Bashan, Y.
(2018). Root growth improvement of
mesquite seedlings and bacterial rhizosphere
and soil community changes are induced by
inoculation with plant growth-promoting
bacteria and promote restoration of eroded
desert soil. Land Degradation &
Development, 29, 1453–1466.
https://doi.org/10.1002/ldr.2904
Hu, C., Lu, W., Mata, A., Nishinari, K., & Fang,
Y. (2021). Ions-induced gelation of alginat:
Mechanisms and applications. International
Journal of Biological Macromolecules, 177,
578–588.
https://doi.org/10.1016/j.ijbiomac.2021.02.08
6
Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K.
F., & Hopwood, D. A. (2000). Practical
Streptomyces genetics. John Innes
Foundation.
Kim, D. E., Lee, E. Y., & Kim, H. S. (2009).
Cloning and characterization of alginat lyase
from a marine bacterium Streptomyces sp.
ALG-5. Marine Biotechnology, 11(1), 10–16.
https://doi.org/10.1007/s10126-008-9114-9
Liu, H., Li, J., & Singh, B. K. (2024). Harnessing
co-evolutionary interactions between plants
and Streptomyces to combat drought stress.
Nature Plants, 10(8), 1159–1171.
https://doi.org/10.1038/s41477-024-01749-1
Mancera-López, M. E., Barrera-Cortés, J.,
Mendoza-Serna, R., Ariza-Castolo, A., &
Santillan, R. (2023). Polymeric encapsulate of
Streptomyces mycelium resistant to
dehydration with air flow at room
temperature. Polymers, 15(1), 207.
https://doi.org/10.3390/polym15010207
Malektaj, H., Drozdov, A. D., & deClaville
Christiansen, J. (2023). Swelling of
homogeneous alginat gels with multi-stimuli
sensitivity. International Journal of
Molecular Sciences, 24(6), 5064.
https://doi.org/10.3390/ijms24065064
Manaila, E., Calina, I. C., Dumitru, M., &
Craciun, G. (2025). Poly(acrylic acid)
natrium alginat superabsorbent hydrogels
synthesized using electron-beam irradiation
Part III: An evaluation of their degradation in
BIOSFER, J.Bio. & Pend.Bio. Vol.1, No.1, Desember 2016
soil.
Molecules,
30,
1126.
https://doi.org/10.3390/molecules30051126
McBride, M. J., & Ensign, J. C. (1987). Effects of
intracellular
trehalose
content
on
Streptomyces griseus spores. Journal of
Bacteriology,
169(11),
4995–5001.
https://doi.org/10.1128/jb.169.11.4995
5001.1987
Nazari, M. T., Schommer, V. A., Braun, J. C. A.,
dos Santos, L. F., Lopes, S. T., Simon, V.,
Machado, B. S., Ferrari, V., Colla, L. M., &
Piccin, J. S. (2023). Using Streptomyces spp.
as plant growth promoters and biocontrol
agents.
Rhizosphere,
27,
100741.
https://doi.org/10.1016/j.rhisph.2023.100741
Retnowati, Y., Kandowangko, N. Y., Katili, A.
S., & Pembengo, W. (2024a). Diversity of
actinomycetes on plant rhizosphere of karst
ecosystem
of
Biodiversitas,
Gorontalo,
25(3),
Indonesia.
907–915.
https://doi.org/10.13057/biodiv/d250301
Retnowati, Y., Kandowangko, N. Y., Katili, A.
S., & Pembengo, W. (2024b). Molecular
identification of rhizospheric Actinomycetes
from karst ecosystems of Gorontalo,
Indonesia, and its seed germination induction
capability of Zea mays var. doti. Biodiversitas,
25(12),
4763–4771.
https://doi.org/10.13057/biodiv/d251212
Retnowati, Y., Katili, A. S., & Kandowangko, N.
Y. (2025c). Bioprospecting and molecular
identification of Streptomyces on karst
ecosystems in the coastal area of Gorontalo,
Indonesia,
as
plant
growth-promoting
rhizobacteria. Biodiversitas, 26(7), 3294
3301.
https://doi.org/10.13057/biodiv/d260721
Rodrigues, D., Rocha-Santos, T. A. P., Sousa, S.,
Gomes, A. M., Pintado, M. M., Malcata, F. X.,
Lobo, J. M. S., Silva, J. P., Costa, P., &
Amaral, M. H. (2011). On the viability of five
probiotic strains when immobilized on various
polymers. International Journal of Dairy
Technology,
64(1),
137–144.
https://doi.org/10.1111/j.1471
0307.2010.00644.x
Rojas-Padilla, J., de-Bashan, L. E., Parra-Cota, F.
I., Rocha-Estrada, J., & de los Santos
Villalobos, S. (2022). Microencapsulation of
Bacillus strains for improving wheat (Triticum
ISSN: xxxx xxx
turgidum subsp. durum) growth and
development.
Plants,
11(21),
2920.
https://doi.org/10.3390/plants11212920
Saberi Riseh, R., Fathi, F., Vatankhah, M., &
Kennedy, J. F. (2025). Alginat supramolecular
for encapsulation of plant biocontrol bacteria:
A review. Carbohydrate Polymers, 358,
123511.
https://doi.org/10.1016/j.carbpol.2025.12351
1
Souza-Alonso, P., Rocha, M., Rocha, I., Ma, Y.,
Freitas, H., & Oliveira, R. S. (2021).
Encapsulation of Pseudomonas libanensis in
alginat beads to sustain bacterial viability and
inoculation of Vigna unguiculata under
drought stress. 3 Biotech, 11, 293.
https://doi.org/10.1007/s13205-021-02818-4
Vargas, P. O., Pereira, N. R., Guimarães, A. O.,
Waldman, W. R., & Pereira, V. R. (2018).
Shrinkage and deformation during convective
drying of kalsium alginat. LWT, 97, 213–222.
https://doi.org/10.1016/j.lwt.2018.06.056
Vassilev, N., Vassileva, M., Martos, V., Garcia
del Moral, L. F., Kowalska, J., Tylkowski, B.,
& Malusá, E. (2020). Formulation of
microbial inoculants by encapsulation in
natural polysaccharides: Focus on beneficial
properties of carrier additives and derivatives.
Frontiers in Plant Science, 11, 270.
Vejan, P., Khadiran, T., Abdullah, R., Ismail, S.,
& Dadrasnia, A. (2019). Encapsulation of
plant growth promoting rhizobacteria—
Prospects and potential in agricultural sector:
A review. Journal of Plant Nutrition, 42,
2600–2623.
Wang, N., Wang, B., Wan, Y., Gao, B., & Rajput,
V. D. (2023). Alginat-based composites as
novel soil conditioners for sustainable
applications in agriculture: A critical review.
Journal of Environmental Management, 348,
119133.
https://doi.org/10.1016/j.jenvman.2023.1191
33
Widyananda, K. S., Purnawati, A., &
Wiyatiningsih, S. (2025). Bioencapsulation of
Streptomyces sp. with natrium alginat for
control Fusarium sp. in shallot plants.
CROPSAVER Journal of Plant Protection, 8(
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Biosfer : Jurnal Biologi dan Pendidikan Biologi
This work is licensed under a Creative Commons Attribution 4.0 International License.
