Uji Perilaku dan Preferensi Area pada Ikan Zebrafish (Danio rerio) yang Diinduksi Stres
DOI:
https://doi.org/10.23969/biosfer.v5i2.3531Keywords:
Danio rerio, perilaku, preferensi, respon stres, stres suhuAbstract
Sistem stres ikan zebra diwakili oleh poros HPI yang serupa dengan poros HPA pada mamalia untuk mengendalikan sirkulasi kortisol sebagai hormon terkait respon stres. Ikan yang berada dalam keadaan stres diduga mempengaruhi perilaku dan preferensi area sesuai dengan parameter yang ditentukan. Tujuan dari penelitian ini adalah menguji efek induksi stres suhu terhadap preferensi area dan perilaku ikan zebra. Penelitian ini dilakukan secara eksperimental dengan parameter uji: preferensi area gelap/terang, preferensi area bawah/atas, dan munculnya perilaku imobilitas dan gerakan tidak menentu. Ikan diamati di tiga akuarium uji secara individual, yaitu akuarium uji gelap/terang, akuarium tangki penyelaman (atas/bawah), dan akuarium uji lapangan terbuka. Data dianalisis secara statistik menggunakan Uji T berpasangan dengan signifikansi P < 0,05. Stres suhu yang diberikan mempengaruhi preferensi area pada ikan zebra, preferensi pada area terang lebih tinggi (rata-rata 33,29 detik/menit) dibandingkan area gelap, preferensi pada area bawah akuarium lebih tinggi (rata-rata 44,96 detik/menit) dibandingkan area bawah, dan perilaku berupa imobilitas dan gerakan tidak menentu ditunjukan oleh ikan zebra sebagai indikasi respon stres. Disimpulkan bahwa perilaku dan preferensi area terpengaruh oleh paparan stresor suhu yang diduga terkait dengan produksi kortisol.Downloads
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